Click image to enlarge. The Makler counting chamber is a simple-to-use device for rapid and accurate sperm count, motility and morphology evaluation, from undiluted specimen. The lower part has a metal base and two handles. In the center of the base there is a flat disc made of optical flat glass on which the sample is placed. The upper part is the cover glass encircled with a metal ring. At the center of its lower surface there is a 1mm2 grid , subdivided into squares, each one of 0.
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Service ivfstore. No additional factors are necessary for calculation. With the aid of a wooden rod or pipette, place a small drop in the centre of the disc area. The sperm heads within the squares of the grid are counted in the same way blood cell are counted in hemocytometer. In the event the number of sperm is substantial, count their number in a strip of 10 squares.
This number represents their concentration in millions per mL. Repeat this count in another strip or two, to determine the average. Alternatively or optionally, it is recommended that the count be made from 2 or 3 other drops of the specimen to increase the reliability of count determination. In the case of oligospermic specimen, it is suggested to count sperm in the entire grid area.
Five zeros are then added to the number counted and the result is the concentration in millions per mL. Do not rinse or soak the chamber in tap water. Dip the brush into water or into noncorrosive antiseptic solution and simply wipe both sides of the glasses.
Then, squeeze the brush and sponge off the remaining water. Finally dry the surface with the lint free lens paper. Avoid touching the tips of the pins as much as possible. The chamber is now ready for reuse.
All content on this site is for reference purposes and is not a substitute for advice from a licensed professional. Always read labels and directions before using a product. Nidacon VitaVitro. All Flasks. All Vials. Oosafe Falcon Nunc. Embryology Training. Contact Customer. Product image 1. Product image 2. You purchased this item on. View this order. Add to Cart. Facebook Twitter Pinterest Fancy Email. Features: The depth of 10 microns eliminates blurring and allows sperm to move freely.
The applied sample is observed in one focal plane. The grid is on the cover glass. This eliminates the need to insert a grid into the microscope eye piece and remove it when not required. Easily cleaned with a non-bleach disinfectant solution. Observation of color fringes at the four contact points, provides a self-controlled test for accuracy. The cover glass can never be raised by the applied sample. Repeated use with complete accuracy without calibration.
Manufactured by state-of-the-art precision engineering. Checked individually by laser beam for precision and accuracy.
Mix the specimen well, taking care to avoid formation of bubbles. Press gently, looking again for the appearance of the color fringes. The drop will spread on the entire area of the disc into a thickness of 10 microns. Lift the chamber by its handles and place it on the stage of the microscope. You may use the chamber grip to fit it properly. Sperm count: The sperm heads within the squares of the grid are counted in the same way blood cell are counted in hemocytometer.
Cleaning and preparation for reuse: Do not rinse or soak the chamber in tap water. Customer Reviews 0 Based on 0 reviews.
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Makler Sperm Counting Chamber Kit
Makler® counting chamber